بررسی توانایی سیانوباکتری Fischerellaambigua ISC67 در تجزیه زیستی نفت خام

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه ایلام

2 دانشگاه مازندران

3 دانشگاه شهید بهشتی تهران، پژوهشکده علوم پایه کاربردی جهاد دانشگاهی

چکیده

هدف اصلی از این مطالعه بررسی توانایی سیانوباکتری ‏ Fischerella ambigua ISC67‎‏ در تجزیه زیستی نفت خام و اثر نفت خام بر پاسخ‌های فیزیولوژیک این سیانوباکتر می‌باشد. در این مطالعه تجربی، سیانوباکتری Fischerella ambiguaISC67 از کلکسیون ریز جلبک‌ها تهیه شد‏. نرخ رشد سیانوباکتری در آزمایه 1% نفت خام در طول موج nm 750 و مقدار کلروفیل در آزمایه های 5/0، 1، 2 و 4% نفت خام در طول موج nm 665 با استفاده از دستگاه اسپکتوفتومتر اندازه‌‌گیری شد. برای تعیین وزن خشک، با استفاده از کاغذ صافی‌های وزن شده، سیانوباکتر از محیط کشت جدا گردید و پس از خشک شدن وزن آنها اندازه‌گیری شد. میزان تجزیه نفت خام به روش آنالیز کروماتوگرافی گازی محاسبه گردید. نتایج نشان داد که رشد این سیانوباکتری در حضور نفت خام تقریباً مشابه با نمونه شاهد افزایش می‌یابد، به طوری که این افزایش نرخ رشد تقریباً مشابه و گاهی کمتر از نمونه شاهد می‌باشد. همچنین با افزایش غلظت نفت خام، میزان وزن خشک سیانوباکتری در آزمایه های 5/0 و 2% نسبت به نمونه شاهد افزایش و میزان کلروفیل در آزمایه‌های مختلف نفت خام کاهش می‌یابد. میانگین تجزیه زیستی هیدرکربن‌های نفتی در آزمایه های روز 14 نسبت به نمونه شاهد 32/42% و در روز 28 به مقدار 21/54% بود. در این مطالعه مشخص شد که سیانوباکتری Fischerella ambigua دارای توانایی بالایی در تجزیه زیستی نفت خام است. لذا نتایج حاصل، بیان‌گر قابلیت کاربرد این سیانوباکتری به‌عنوان شاخصی جهت رفع آلودگی‌های نفتی در مناطق آلوده می‌باشد.‏
 

کلیدواژه‌ها


عنوان مقاله [English]

The Potential of Cyanobacterium Fischerella Ambigua ISC67 ‎in Biodegradation of Crude Oil

نویسنده [English]

  • Moein Safari 1
1
2
3
چکیده [English]

The main objective of this study is to investigate the potential of cyanobacterium Fischerella ambigua ISC67‎ in the biodegradation of crude oil and evaluation of oil effect on physiological responses. In this experimental study, the cyanobacterium Fischerella ambigua ‎ ISC67 was obtained from the algal ‎culture collection. The growth rate of cyanobacteria in 1% treatment and control sample of crude oil measured at ‎‎750 nm and chlorophyll content in 0.5, 1, 2, and 4% treatments of crude oil measured at h nm ‎‎665nm wavelength using a spectrophotometer. For measuring of dry weight, firstly‎ using weighed ‎filter paper cyanobacteria were isolated from the culture medium and their weights were measured ‎after drying. ‎The rate of biodegradation of crude oil analyzed by gas chromatography. The results showed that the growth of this ‎cyanobacterium in the presence  of crude oil is almost same as of control sample.‎ So that these growth rates are nearly equal and sometimes lower than the control sample. Also, with increasing concentrations of crude oil, dry weight of this cyanobacterium in 0.5% and 2% treatments increased compared to the control sample and it chlorophyll rate in the various treatments of crude is reduced The average of petroleum hydrocarbon biodegradation in 14 days of treatment compared to the control sample was at a rate of 42.32 % and and 28 days was 54.21% respectively. In this study it was found that cyanobacterium Fischerella ambigua ISC67‎ has great potential in biodegradation of crude oil. Therefore, these results indicate the potential of this cyanobacterium for its use as an indicator to eliminate pollution in contaminated areas.
 

کلیدواژه‌ها [English]

  • Cyanobacteria
  • Biodegradation
  • Crude Oil
  • Chlorophyll
  • Gas Chromatography
 
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